Composite

Part:BBa_K1460002:Design

Designed by: Eric Holmes   Group: iGEM14_Cornell   (2014-08-14)


RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 706
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 118


Design Notes

The part contains a KpnI cut site between the biobrick prefix and the RBS that is a result of removing the T7 promoter from part BBa_K1460001.


Source

This part is BBa_K1460001 with the T7 promoter removed.

References